anti vasointestinal peptide Search Results


90
ImmunoStar inc rabbit anti-vasointestinal peptide
Rabbit Anti Vasointestinal Peptide, supplied by ImmunoStar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti-vasointestinal peptide - by Bioz Stars, 2026-11
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ImmunoStar inc rabbit vasoactive intestinal peptide (rvip)
Name, target, source, and dilution of primary antibodies
Rabbit Vasoactive Intestinal Peptide (Rvip), supplied by ImmunoStar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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93
Cusabio vasoactive intestinal peptide vip type 1 receptor
Vasoactive intestinal peptide receptors in mice colon during DSS colitis with sleep fragmentation. ( A ) Immunohistochemical (IHC) staining of <t>VPAC1</t> and VPAC2 in colon sections. Upper part: expression of VPAC1 in colon tissue of different groups at 200× & 400× magnification. Comparison of the VPAC1 in five groups was shown in ( B ) right upper panel. ( A ) Lower part: expression of VPAC2 in colon tissue of different groups at 200× & 400× magnification. Comparison of the VPAC2 in five groups was shown in ( B ) right middle panel. Comparison of VPAC1/VPAC2 ratios by sleep fragmentation in normal and inflamed state was shown in ( B ) right lower panel. Quantification of area percentage of IHC staining by true color image analysis with the application of adjusted thresholds. ( C ) Western blot analysis of VPAC2 and β-actin (loading control) in colon homogenates. Right graph indicates quantification relative to β-actin. * Stands for a result of five groups comparison (represented by bold line segments with endpoints) The uncropped western blot figures were presented in . The densitometry readings/intensity ratio of VPAC2 in western blots in DSS-colitis mice with sleep fragmentation were presented in . ✠, ✟, #, $, & and € represent the results of control group versus DSS group, DSS group versus DSS + SF group, DSS + SF group versus DSS + SF + EA group, control group versus DSS + SF group, control group versus DSS + SF + EA group, and control group versus SF group, respectively (represented as a thin line without endpoints).*, ✠, ✟, #, $, &, €, p < 0.05; **, ✠✠, ✟✟, ##, $$, &&, €€ p < 0.01; ***, p < 0.001; ns, no significance. Data were presented as mean ± SEM of repeated adjusted thresholds in each group. VPAC1, vasoactive intestinal peptide (VIP) <t>type</t> <t>1</t> receptor; VPAC2, vasoactive intestinal peptide (VIP) type 2 receptor; DSS, dextran sodium sulfate; SF, sleep fragmentation; EA, electroacupuncture.
Vasoactive Intestinal Peptide Vip Type 1 Receptor, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+vasointestinal+peptide/Rabbit+anti-+VIPR1+Polyclonal+Antibody/pmc09311573-128-6-15
Average 93 stars, based on 1 article reviews
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90
Biomol GmbH antibodies against vasointestinal peptide (vip)
Vasoactive intestinal peptide receptors in mice colon during DSS colitis with sleep fragmentation. ( A ) Immunohistochemical (IHC) staining of <t>VPAC1</t> and VPAC2 in colon sections. Upper part: expression of VPAC1 in colon tissue of different groups at 200× & 400× magnification. Comparison of the VPAC1 in five groups was shown in ( B ) right upper panel. ( A ) Lower part: expression of VPAC2 in colon tissue of different groups at 200× & 400× magnification. Comparison of the VPAC2 in five groups was shown in ( B ) right middle panel. Comparison of VPAC1/VPAC2 ratios by sleep fragmentation in normal and inflamed state was shown in ( B ) right lower panel. Quantification of area percentage of IHC staining by true color image analysis with the application of adjusted thresholds. ( C ) Western blot analysis of VPAC2 and β-actin (loading control) in colon homogenates. Right graph indicates quantification relative to β-actin. * Stands for a result of five groups comparison (represented by bold line segments with endpoints) The uncropped western blot figures were presented in . The densitometry readings/intensity ratio of VPAC2 in western blots in DSS-colitis mice with sleep fragmentation were presented in . ✠, ✟, #, $, & and € represent the results of control group versus DSS group, DSS group versus DSS + SF group, DSS + SF group versus DSS + SF + EA group, control group versus DSS + SF group, control group versus DSS + SF + EA group, and control group versus SF group, respectively (represented as a thin line without endpoints).*, ✠, ✟, #, $, &, €, p < 0.05; **, ✠✠, ✟✟, ##, $$, &&, €€ p < 0.01; ***, p < 0.001; ns, no significance. Data were presented as mean ± SEM of repeated adjusted thresholds in each group. VPAC1, vasoactive intestinal peptide (VIP) <t>type</t> <t>1</t> receptor; VPAC2, vasoactive intestinal peptide (VIP) type 2 receptor; DSS, dextran sodium sulfate; SF, sleep fragmentation; EA, electroacupuncture.
Antibodies Against Vasointestinal Peptide (Vip), supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+vasointestinal+peptide/antibodies+against+vasointestinal+peptide++vip+/pmc07756863-33-24-28
Average 90 stars, based on 1 article reviews
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93
Aviva Systems rabbit polyclonal anti human vasoactive intestinal peptide
Vasoactive intestinal peptide receptors in mice colon during DSS colitis with sleep fragmentation. ( A ) Immunohistochemical (IHC) staining of <t>VPAC1</t> and VPAC2 in colon sections. Upper part: expression of VPAC1 in colon tissue of different groups at 200× & 400× magnification. Comparison of the VPAC1 in five groups was shown in ( B ) right upper panel. ( A ) Lower part: expression of VPAC2 in colon tissue of different groups at 200× & 400× magnification. Comparison of the VPAC2 in five groups was shown in ( B ) right middle panel. Comparison of VPAC1/VPAC2 ratios by sleep fragmentation in normal and inflamed state was shown in ( B ) right lower panel. Quantification of area percentage of IHC staining by true color image analysis with the application of adjusted thresholds. ( C ) Western blot analysis of VPAC2 and β-actin (loading control) in colon homogenates. Right graph indicates quantification relative to β-actin. * Stands for a result of five groups comparison (represented by bold line segments with endpoints) The uncropped western blot figures were presented in . The densitometry readings/intensity ratio of VPAC2 in western blots in DSS-colitis mice with sleep fragmentation were presented in . ✠, ✟, #, $, & and € represent the results of control group versus DSS group, DSS group versus DSS + SF group, DSS + SF group versus DSS + SF + EA group, control group versus DSS + SF group, control group versus DSS + SF + EA group, and control group versus SF group, respectively (represented as a thin line without endpoints).*, ✠, ✟, #, $, &, €, p < 0.05; **, ✠✠, ✟✟, ##, $$, &&, €€ p < 0.01; ***, p < 0.001; ns, no significance. Data were presented as mean ± SEM of repeated adjusted thresholds in each group. VPAC1, vasoactive intestinal peptide (VIP) <t>type</t> <t>1</t> receptor; VPAC2, vasoactive intestinal peptide (VIP) type 2 receptor; DSS, dextran sodium sulfate; SF, sleep fragmentation; EA, electroacupuncture.
Rabbit Polyclonal Anti Human Vasoactive Intestinal Peptide, supplied by Aviva Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+vasointestinal+peptide/Anti-CD147%2FEmmprin+Rabbit+Polyclonal+Antibody+(OAAI00017)/pm28642706-70-76-84
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rabbit polyclonal anti human vasoactive intestinal peptide - by Bioz Stars, 2026-11
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92
R&D Systems anti vasointestinal peptide
Vasoactive intestinal peptide receptors in mice colon during DSS colitis with sleep fragmentation. ( A ) Immunohistochemical (IHC) staining of <t>VPAC1</t> and VPAC2 in colon sections. Upper part: expression of VPAC1 in colon tissue of different groups at 200× & 400× magnification. Comparison of the VPAC1 in five groups was shown in ( B ) right upper panel. ( A ) Lower part: expression of VPAC2 in colon tissue of different groups at 200× & 400× magnification. Comparison of the VPAC2 in five groups was shown in ( B ) right middle panel. Comparison of VPAC1/VPAC2 ratios by sleep fragmentation in normal and inflamed state was shown in ( B ) right lower panel. Quantification of area percentage of IHC staining by true color image analysis with the application of adjusted thresholds. ( C ) Western blot analysis of VPAC2 and β-actin (loading control) in colon homogenates. Right graph indicates quantification relative to β-actin. * Stands for a result of five groups comparison (represented by bold line segments with endpoints) The uncropped western blot figures were presented in . The densitometry readings/intensity ratio of VPAC2 in western blots in DSS-colitis mice with sleep fragmentation were presented in . ✠, ✟, #, $, & and € represent the results of control group versus DSS group, DSS group versus DSS + SF group, DSS + SF group versus DSS + SF + EA group, control group versus DSS + SF group, control group versus DSS + SF + EA group, and control group versus SF group, respectively (represented as a thin line without endpoints).*, ✠, ✟, #, $, &, €, p < 0.05; **, ✠✠, ✟✟, ##, $$, &&, €€ p < 0.01; ***, p < 0.001; ns, no significance. Data were presented as mean ± SEM of repeated adjusted thresholds in each group. VPAC1, vasoactive intestinal peptide (VIP) <t>type</t> <t>1</t> receptor; VPAC2, vasoactive intestinal peptide (VIP) type 2 receptor; DSS, dextran sodium sulfate; SF, sleep fragmentation; EA, electroacupuncture.
Anti Vasointestinal Peptide, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+vasointestinal+peptide/Human+VIP+Antibody/bio_rxiv__2024__10__26__620416-145-47-54
Average 92 stars, based on 1 article reviews
anti vasointestinal peptide - by Bioz Stars, 2026-11
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90
Swant anti-parvalbumin (pv
Vasoactive intestinal peptide receptors in mice colon during DSS colitis with sleep fragmentation. ( A ) Immunohistochemical (IHC) staining of <t>VPAC1</t> and VPAC2 in colon sections. Upper part: expression of VPAC1 in colon tissue of different groups at 200× & 400× magnification. Comparison of the VPAC1 in five groups was shown in ( B ) right upper panel. ( A ) Lower part: expression of VPAC2 in colon tissue of different groups at 200× & 400× magnification. Comparison of the VPAC2 in five groups was shown in ( B ) right middle panel. Comparison of VPAC1/VPAC2 ratios by sleep fragmentation in normal and inflamed state was shown in ( B ) right lower panel. Quantification of area percentage of IHC staining by true color image analysis with the application of adjusted thresholds. ( C ) Western blot analysis of VPAC2 and β-actin (loading control) in colon homogenates. Right graph indicates quantification relative to β-actin. * Stands for a result of five groups comparison (represented by bold line segments with endpoints) The uncropped western blot figures were presented in . The densitometry readings/intensity ratio of VPAC2 in western blots in DSS-colitis mice with sleep fragmentation were presented in . ✠, ✟, #, $, & and € represent the results of control group versus DSS group, DSS group versus DSS + SF group, DSS + SF group versus DSS + SF + EA group, control group versus DSS + SF group, control group versus DSS + SF + EA group, and control group versus SF group, respectively (represented as a thin line without endpoints).*, ✠, ✟, #, $, &, €, p < 0.05; **, ✠✠, ✟✟, ##, $$, &&, €€ p < 0.01; ***, p < 0.001; ns, no significance. Data were presented as mean ± SEM of repeated adjusted thresholds in each group. VPAC1, vasoactive intestinal peptide (VIP) <t>type</t> <t>1</t> receptor; VPAC2, vasoactive intestinal peptide (VIP) type 2 receptor; DSS, dextran sodium sulfate; SF, sleep fragmentation; EA, electroacupuncture.
Anti Parvalbumin (Pv, supplied by Swant, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Swant anti-calretinin (cr
Vasoactive intestinal peptide receptors in mice colon during DSS colitis with sleep fragmentation. ( A ) Immunohistochemical (IHC) staining of <t>VPAC1</t> and VPAC2 in colon sections. Upper part: expression of VPAC1 in colon tissue of different groups at 200× & 400× magnification. Comparison of the VPAC1 in five groups was shown in ( B ) right upper panel. ( A ) Lower part: expression of VPAC2 in colon tissue of different groups at 200× & 400× magnification. Comparison of the VPAC2 in five groups was shown in ( B ) right middle panel. Comparison of VPAC1/VPAC2 ratios by sleep fragmentation in normal and inflamed state was shown in ( B ) right lower panel. Quantification of area percentage of IHC staining by true color image analysis with the application of adjusted thresholds. ( C ) Western blot analysis of VPAC2 and β-actin (loading control) in colon homogenates. Right graph indicates quantification relative to β-actin. * Stands for a result of five groups comparison (represented by bold line segments with endpoints) The uncropped western blot figures were presented in . The densitometry readings/intensity ratio of VPAC2 in western blots in DSS-colitis mice with sleep fragmentation were presented in . ✠, ✟, #, $, & and € represent the results of control group versus DSS group, DSS group versus DSS + SF group, DSS + SF group versus DSS + SF + EA group, control group versus DSS + SF group, control group versus DSS + SF + EA group, and control group versus SF group, respectively (represented as a thin line without endpoints).*, ✠, ✟, #, $, &, €, p < 0.05; **, ✠✠, ✟✟, ##, $$, &&, €€ p < 0.01; ***, p < 0.001; ns, no significance. Data were presented as mean ± SEM of repeated adjusted thresholds in each group. VPAC1, vasoactive intestinal peptide (VIP) <t>type</t> <t>1</t> receptor; VPAC2, vasoactive intestinal peptide (VIP) type 2 receptor; DSS, dextran sodium sulfate; SF, sleep fragmentation; EA, electroacupuncture.
Anti Calretinin (Cr, supplied by Swant, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+vasointestinal+peptide/calretinin+antibody/pm35486347-208-24-26
Average 90 stars, based on 1 article reviews
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90
Swant anti-parvalnumin (pv
Vasoactive intestinal peptide receptors in mice colon during DSS colitis with sleep fragmentation. ( A ) Immunohistochemical (IHC) staining of <t>VPAC1</t> and VPAC2 in colon sections. Upper part: expression of VPAC1 in colon tissue of different groups at 200× & 400× magnification. Comparison of the VPAC1 in five groups was shown in ( B ) right upper panel. ( A ) Lower part: expression of VPAC2 in colon tissue of different groups at 200× & 400× magnification. Comparison of the VPAC2 in five groups was shown in ( B ) right middle panel. Comparison of VPAC1/VPAC2 ratios by sleep fragmentation in normal and inflamed state was shown in ( B ) right lower panel. Quantification of area percentage of IHC staining by true color image analysis with the application of adjusted thresholds. ( C ) Western blot analysis of VPAC2 and β-actin (loading control) in colon homogenates. Right graph indicates quantification relative to β-actin. * Stands for a result of five groups comparison (represented by bold line segments with endpoints) The uncropped western blot figures were presented in . The densitometry readings/intensity ratio of VPAC2 in western blots in DSS-colitis mice with sleep fragmentation were presented in . ✠, ✟, #, $, & and € represent the results of control group versus DSS group, DSS group versus DSS + SF group, DSS + SF group versus DSS + SF + EA group, control group versus DSS + SF group, control group versus DSS + SF + EA group, and control group versus SF group, respectively (represented as a thin line without endpoints).*, ✠, ✟, #, $, &, €, p < 0.05; **, ✠✠, ✟✟, ##, $$, &&, €€ p < 0.01; ***, p < 0.001; ns, no significance. Data were presented as mean ± SEM of repeated adjusted thresholds in each group. VPAC1, vasoactive intestinal peptide (VIP) <t>type</t> <t>1</t> receptor; VPAC2, vasoactive intestinal peptide (VIP) type 2 receptor; DSS, dextran sodium sulfate; SF, sleep fragmentation; EA, electroacupuncture.
Anti Parvalnumin (Pv, supplied by Swant, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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MBL Life science mouse anti-reelin
Vasoactive intestinal peptide receptors in mice colon during DSS colitis with sleep fragmentation. ( A ) Immunohistochemical (IHC) staining of <t>VPAC1</t> and VPAC2 in colon sections. Upper part: expression of VPAC1 in colon tissue of different groups at 200× & 400× magnification. Comparison of the VPAC1 in five groups was shown in ( B ) right upper panel. ( A ) Lower part: expression of VPAC2 in colon tissue of different groups at 200× & 400× magnification. Comparison of the VPAC2 in five groups was shown in ( B ) right middle panel. Comparison of VPAC1/VPAC2 ratios by sleep fragmentation in normal and inflamed state was shown in ( B ) right lower panel. Quantification of area percentage of IHC staining by true color image analysis with the application of adjusted thresholds. ( C ) Western blot analysis of VPAC2 and β-actin (loading control) in colon homogenates. Right graph indicates quantification relative to β-actin. * Stands for a result of five groups comparison (represented by bold line segments with endpoints) The uncropped western blot figures were presented in . The densitometry readings/intensity ratio of VPAC2 in western blots in DSS-colitis mice with sleep fragmentation were presented in . ✠, ✟, #, $, & and € represent the results of control group versus DSS group, DSS group versus DSS + SF group, DSS + SF group versus DSS + SF + EA group, control group versus DSS + SF group, control group versus DSS + SF + EA group, and control group versus SF group, respectively (represented as a thin line without endpoints).*, ✠, ✟, #, $, &, €, p < 0.05; **, ✠✠, ✟✟, ##, $$, &&, €€ p < 0.01; ***, p < 0.001; ns, no significance. Data were presented as mean ± SEM of repeated adjusted thresholds in each group. VPAC1, vasoactive intestinal peptide (VIP) <t>type</t> <t>1</t> receptor; VPAC2, vasoactive intestinal peptide (VIP) type 2 receptor; DSS, dextran sodium sulfate; SF, sleep fragmentation; EA, electroacupuncture.
Mouse Anti Reelin, supplied by MBL Life science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+vasointestinal+peptide/mouse+anti+reelin/pmc04281262-214-13-15
Average 90 stars, based on 1 article reviews
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90
Swant anti-calbindin
Vasoactive intestinal peptide receptors in mice colon during DSS colitis with sleep fragmentation. ( A ) Immunohistochemical (IHC) staining of <t>VPAC1</t> and VPAC2 in colon sections. Upper part: expression of VPAC1 in colon tissue of different groups at 200× & 400× magnification. Comparison of the VPAC1 in five groups was shown in ( B ) right upper panel. ( A ) Lower part: expression of VPAC2 in colon tissue of different groups at 200× & 400× magnification. Comparison of the VPAC2 in five groups was shown in ( B ) right middle panel. Comparison of VPAC1/VPAC2 ratios by sleep fragmentation in normal and inflamed state was shown in ( B ) right lower panel. Quantification of area percentage of IHC staining by true color image analysis with the application of adjusted thresholds. ( C ) Western blot analysis of VPAC2 and β-actin (loading control) in colon homogenates. Right graph indicates quantification relative to β-actin. * Stands for a result of five groups comparison (represented by bold line segments with endpoints) The uncropped western blot figures were presented in . The densitometry readings/intensity ratio of VPAC2 in western blots in DSS-colitis mice with sleep fragmentation were presented in . ✠, ✟, #, $, & and € represent the results of control group versus DSS group, DSS group versus DSS + SF group, DSS + SF group versus DSS + SF + EA group, control group versus DSS + SF group, control group versus DSS + SF + EA group, and control group versus SF group, respectively (represented as a thin line without endpoints).*, ✠, ✟, #, $, &, €, p < 0.05; **, ✠✠, ✟✟, ##, $$, &&, €€ p < 0.01; ***, p < 0.001; ns, no significance. Data were presented as mean ± SEM of repeated adjusted thresholds in each group. VPAC1, vasoactive intestinal peptide (VIP) <t>type</t> <t>1</t> receptor; VPAC2, vasoactive intestinal peptide (VIP) type 2 receptor; DSS, dextran sodium sulfate; SF, sleep fragmentation; EA, electroacupuncture.
Anti Calbindin, supplied by Swant, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Swant rabbit anti-parvalbumin (pv)
Vasoactive intestinal peptide receptors in mice colon during DSS colitis with sleep fragmentation. ( A ) Immunohistochemical (IHC) staining of <t>VPAC1</t> and VPAC2 in colon sections. Upper part: expression of VPAC1 in colon tissue of different groups at 200× & 400× magnification. Comparison of the VPAC1 in five groups was shown in ( B ) right upper panel. ( A ) Lower part: expression of VPAC2 in colon tissue of different groups at 200× & 400× magnification. Comparison of the VPAC2 in five groups was shown in ( B ) right middle panel. Comparison of VPAC1/VPAC2 ratios by sleep fragmentation in normal and inflamed state was shown in ( B ) right lower panel. Quantification of area percentage of IHC staining by true color image analysis with the application of adjusted thresholds. ( C ) Western blot analysis of VPAC2 and β-actin (loading control) in colon homogenates. Right graph indicates quantification relative to β-actin. * Stands for a result of five groups comparison (represented by bold line segments with endpoints) The uncropped western blot figures were presented in . The densitometry readings/intensity ratio of VPAC2 in western blots in DSS-colitis mice with sleep fragmentation were presented in . ✠, ✟, #, $, & and € represent the results of control group versus DSS group, DSS group versus DSS + SF group, DSS + SF group versus DSS + SF + EA group, control group versus DSS + SF group, control group versus DSS + SF + EA group, and control group versus SF group, respectively (represented as a thin line without endpoints).*, ✠, ✟, #, $, &, €, p < 0.05; **, ✠✠, ✟✟, ##, $$, &&, €€ p < 0.01; ***, p < 0.001; ns, no significance. Data were presented as mean ± SEM of repeated adjusted thresholds in each group. VPAC1, vasoactive intestinal peptide (VIP) <t>type</t> <t>1</t> receptor; VPAC2, vasoactive intestinal peptide (VIP) type 2 receptor; DSS, dextran sodium sulfate; SF, sleep fragmentation; EA, electroacupuncture.
Rabbit Anti Parvalbumin (Pv), supplied by Swant, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Name, target, source, and dilution of primary antibodies

Journal: Annals of Clinical and Translational Neurology

Article Title: Unusual Stüve-Wiedemann syndrome with complete maternal chromosome 5 isodisomy

doi: 10.1002/acn3.126

Figure Lengend Snippet: Name, target, source, and dilution of primary antibodies

Article Snippet: Rabbit vasoactive intestinal peptide (rVIP) , Cholinergic nerve fibers , Immunostar (Hudson WI, US) , 1:1000.

Techniques: Marker

Vasoactive intestinal peptide receptors in mice colon during DSS colitis with sleep fragmentation. ( A ) Immunohistochemical (IHC) staining of VPAC1 and VPAC2 in colon sections. Upper part: expression of VPAC1 in colon tissue of different groups at 200× & 400× magnification. Comparison of the VPAC1 in five groups was shown in ( B ) right upper panel. ( A ) Lower part: expression of VPAC2 in colon tissue of different groups at 200× & 400× magnification. Comparison of the VPAC2 in five groups was shown in ( B ) right middle panel. Comparison of VPAC1/VPAC2 ratios by sleep fragmentation in normal and inflamed state was shown in ( B ) right lower panel. Quantification of area percentage of IHC staining by true color image analysis with the application of adjusted thresholds. ( C ) Western blot analysis of VPAC2 and β-actin (loading control) in colon homogenates. Right graph indicates quantification relative to β-actin. * Stands for a result of five groups comparison (represented by bold line segments with endpoints) The uncropped western blot figures were presented in . The densitometry readings/intensity ratio of VPAC2 in western blots in DSS-colitis mice with sleep fragmentation were presented in . ✠, ✟, #, $, & and € represent the results of control group versus DSS group, DSS group versus DSS + SF group, DSS + SF group versus DSS + SF + EA group, control group versus DSS + SF group, control group versus DSS + SF + EA group, and control group versus SF group, respectively (represented as a thin line without endpoints).*, ✠, ✟, #, $, &, €, p < 0.05; **, ✠✠, ✟✟, ##, $$, &&, €€ p < 0.01; ***, p < 0.001; ns, no significance. Data were presented as mean ± SEM of repeated adjusted thresholds in each group. VPAC1, vasoactive intestinal peptide (VIP) type 1 receptor; VPAC2, vasoactive intestinal peptide (VIP) type 2 receptor; DSS, dextran sodium sulfate; SF, sleep fragmentation; EA, electroacupuncture.

Journal: Biology

Article Title: Alterations in Gut Microbiota and Upregulations of VPAC2 and Intestinal Tight Junctions Correlate with Anti-Inflammatory Effects of Electroacupuncture in Colitis Mice with Sleep Fragmentation

doi: 10.3390/biology11070962

Figure Lengend Snippet: Vasoactive intestinal peptide receptors in mice colon during DSS colitis with sleep fragmentation. ( A ) Immunohistochemical (IHC) staining of VPAC1 and VPAC2 in colon sections. Upper part: expression of VPAC1 in colon tissue of different groups at 200× & 400× magnification. Comparison of the VPAC1 in five groups was shown in ( B ) right upper panel. ( A ) Lower part: expression of VPAC2 in colon tissue of different groups at 200× & 400× magnification. Comparison of the VPAC2 in five groups was shown in ( B ) right middle panel. Comparison of VPAC1/VPAC2 ratios by sleep fragmentation in normal and inflamed state was shown in ( B ) right lower panel. Quantification of area percentage of IHC staining by true color image analysis with the application of adjusted thresholds. ( C ) Western blot analysis of VPAC2 and β-actin (loading control) in colon homogenates. Right graph indicates quantification relative to β-actin. * Stands for a result of five groups comparison (represented by bold line segments with endpoints) The uncropped western blot figures were presented in . The densitometry readings/intensity ratio of VPAC2 in western blots in DSS-colitis mice with sleep fragmentation were presented in . ✠, ✟, #, $, & and € represent the results of control group versus DSS group, DSS group versus DSS + SF group, DSS + SF group versus DSS + SF + EA group, control group versus DSS + SF group, control group versus DSS + SF + EA group, and control group versus SF group, respectively (represented as a thin line without endpoints).*, ✠, ✟, #, $, &, €, p < 0.05; **, ✠✠, ✟✟, ##, $$, &&, €€ p < 0.01; ***, p < 0.001; ns, no significance. Data were presented as mean ± SEM of repeated adjusted thresholds in each group. VPAC1, vasoactive intestinal peptide (VIP) type 1 receptor; VPAC2, vasoactive intestinal peptide (VIP) type 2 receptor; DSS, dextran sodium sulfate; SF, sleep fragmentation; EA, electroacupuncture.

Article Snippet: In addition, the role of the vasoactive intestinal peptide (VIP) type 1 receptor (VIPR1/VPAC1, CSB-PA052529, Cusabio, Houston, TX, USA) and type 2 receptor (VIPR2/VPAC2, A03768, Boster, Pleasanton, CA, USA) under EA were clarified by immunohistochemical staining.

Techniques: Immunohistochemical staining, Immunohistochemistry, Expressing, Western Blot